Multipotent adult progenitor cell isolation and culture procedures

April Breyer; Nima Estharabadi; Masayuki Oki; Fernando Ulloa; Molly Nelson-Holte; Laura Lien; Yuehua Jiang

doi:10.1016/j.exphem.2006.07.013

Multipotent adult progenitor cell isolation and culture procedures

Exp Hematol. 2006 Nov;34(11):1596-601. doi: 10.1016/j.exphem.2006.07.013.

Authors

April Breyer¹, Nima Estharabadi, Masayuki Oki, Fernando Ulloa, Molly Nelson-Holte, Laura Lien, Yuehua Jiang

Affiliation

¹ University of Minnesota, Stem Cell Institute, Minneapolis, MN 55455, USA.

PMID: 17046581
DOI: 10.1016/j.exphem.2006.07.013

Abstract

We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed.

MeSH terms

Animals
Cell Culture Techniques / methods
Cell Differentiation / drug effects
Cell Differentiation / immunology
Cell Separation / methods*
Cells, Cultured
Culture Media / pharmacology
Freezing
Immunohistochemistry
Mice
Multipotent Stem Cells / cytology*
Multipotent Stem Cells / drug effects
Multipotent Stem Cells / immunology
Phenotype
Quality Control
Rats
Tissue Preservation

Substances

Culture Media