Abstract
In Escherichia coli, the ATP-dependent DNA translocase FtsK transports DNA across the site of cell division and activates recombination by the XerCD recombinases at a specific site on the chromosome, dif, to ensure the last stages of chromosome segregation. DNA transport by FtsK is oriented by 8-base-pair asymmetric sequences ('KOPS'). Here we provide evidence that KOPS promote FtsK loading on DNA and that translocation is oriented at this step.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Base Sequence
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Chromosomes, Bacterial / metabolism
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DNA, Bacterial / chemistry
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DNA, Bacterial / metabolism*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism
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Escherichia coli / enzymology*
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Escherichia coli / metabolism
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Escherichia coli Proteins / chemistry*
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Escherichia coli Proteins / metabolism*
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Integrases / metabolism
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Membrane Proteins / chemistry*
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Membrane Proteins / metabolism*
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Models, Molecular
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Protein Structure, Tertiary
Substances
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DNA, Bacterial
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DNA-Binding Proteins
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Escherichia coli Proteins
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FtsK protein, E coli
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Membrane Proteins
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XerC protein, E coli
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Adenosine Triphosphate
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Integrases
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XerD protein, E coli