Analysis of antibody response in humans to the type A OspC loop 5 domain and assessment of the potential utility of the loop 5 epitope in Lyme disease vaccine development

Eric L Buckles; Christopher G Earnhart; Richard T Marconi

doi:10.1128/CVI.00099-06

Analysis of antibody response in humans to the type A OspC loop 5 domain and assessment of the potential utility of the loop 5 epitope in Lyme disease vaccine development

Clin Vaccine Immunol. 2006 Oct;13(10):1162-5. doi: 10.1128/CVI.00099-06.

Authors

Eric L Buckles¹, Christopher G Earnhart, Richard T Marconi

Affiliation

¹ Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, 1112 E. Clay St., McGuire Hall, Richmond, VA 23298-0678, USA.

Abstract

The OspC protein of Borrelia burgdorferi is an immunodominant antigen. Here we demonstrate that the loop 5 domain of type A OspC is surface exposed, elicits bactericidal antibody in mice, and is antigenic in humans. The data suggest that loop 5 may be suitable for inclusion in a polyvalent, chimeric OspC vaccinogen.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Antibodies, Bacterial / biosynthesis*
Antigens, Bacterial / chemistry*
Antigens, Bacterial / immunology*
Bacterial Outer Membrane Proteins / chemistry*
Bacterial Outer Membrane Proteins / immunology*
Epitopes / immunology*
Humans
Lyme Disease / immunology*
Lyme Disease / microbiology
Lyme Disease Vaccines / chemical synthesis
Lyme Disease Vaccines / immunology*
Molecular Sequence Data
Protein Structure, Tertiary

Substances

Antibodies, Bacterial
Antigens, Bacterial
Bacterial Outer Membrane Proteins
Epitopes
Lyme Disease Vaccines
OspC protein