Abstract
The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and the ratio of alleles determine the detection sensitivity of the probe systems.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antitubercular Agents / pharmacology
-
DNA, Bacterial / genetics
-
Drug Resistance, Bacterial / genetics*
-
Fluorescence Resonance Energy Transfer / methods*
-
Mycobacterium tuberculosis / drug effects*
-
Mycobacterium tuberculosis / genetics*
-
Nucleic Acid Probes*
-
Polymerase Chain Reaction / methods*
-
Polymorphism, Single Nucleotide
Substances
-
Antitubercular Agents
-
DNA, Bacterial
-
Nucleic Acid Probes