After the segregation of chromosomes, animal and plant cells build a central spindle (midbody) and a phragmoplast, respectively, that are mainly composed of aligned microtubules and microfilaments. These microtubule-based structures are highly dynamic and play an essential role in cytokinesis. Recent studies using model organisms have shed light on the involvement of common molecules in the regulatory mechanisms of cytokinesis, including microtubule dynamics, in a variety of species. Among these molecules, members of the MAP65 protein family, a microtubule-associated protein family, appear to be key regulators of both the maintenance and dynamics of central spindles and phragmoplasts.