Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis

Yang Ming-Ming; Zhang Wei-Wei; Zhang Xi-Feng; Cen Pei-Lin

doi:10.1007/s10529-006-9146-z

Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis

Biotechnol Lett. 2006 Nov;28(21):1713-8. doi: 10.1007/s10529-006-9146-z. Epub 2006 Aug 3.

Authors

Yang Ming-Ming¹, Zhang Wei-Wei, Zhang Xi-Feng, Cen Pei-Lin

Affiliation

¹ Institute of Biochemical Engineering, College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, P.R. China.

PMID: 17001500
DOI: 10.1007/s10529-006-9146-z

Abstract

A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics*
Bacillus subtilis / metabolism
Bacterial Proteins / metabolism
Enzyme Induction
Gene Expression Regulation, Bacterial / genetics*
Genetic Vectors / biosynthesis
Genetic Vectors / genetics*
Hemeproteins / metabolism
Maltose / metabolism
Operon / genetics*
Promoter Regions, Genetic
Transaminases / metabolism
Transformation, Bacterial
beta-Galactosidase / metabolism

Substances

Bacterial Proteins
Hemeproteins
Vgb protein, Bacteria
Maltose
Transaminases
BioA protein, bacteria
beta-Galactosidase