Abstract
The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influence expression when cells were growing in a chemostat with succinate at maximum rate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biodegradation, Environmental
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Culture Media
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Gene Expression Regulation, Bacterial*
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Mutation
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics
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Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism*
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Pseudomonas putida / genetics
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Pseudomonas putida / growth & development*
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Pseudomonas putida / metabolism
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Succinates / metabolism
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Toluene / metabolism*
Substances
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Bacterial Proteins
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Culture Media
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PtsN protein, Pseudomonas putida
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Repressor Proteins
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Succinates
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crc protein, Pseudomonas
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Toluene
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Phosphoenolpyruvate Sugar Phosphotransferase System