Abstract
Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibody Formation
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Aspartic Acid Endopeptidases / genetics
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Aspartic Acid Endopeptidases / immunology*
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Blotting, Western / veterinary
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Cattle
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Cattle Diseases / immunology
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Cattle Diseases / parasitology*
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Cattle Diseases / prevention & control*
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Enzyme Precursors / genetics
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Enzyme Precursors / immunology*
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Enzyme-Linked Immunosorbent Assay / veterinary
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Female
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Immunoglobulin G / blood
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Ixodidae / immunology*
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Tick Infestations / immunology
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Tick Infestations / parasitology
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Tick Infestations / prevention & control*
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Tick Infestations / veterinary*
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Vaccination / veterinary*
Substances
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Enzyme Precursors
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Immunoglobulin G
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Recombinant Proteins
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Aspartic Acid Endopeptidases
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Boophilus yolk pro-cathepsin