[Large-scale production of monoclonal antibodies using hollow fiber bioreactor]

B S Chen; G C Chen; J H Hsieh; M H Meng; T F Tsai; J J Liu; J H Huang; T H Chang

[Large-scale production of monoclonal antibodies using hollow fiber bioreactor]

Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1990 Feb;23(1):75-88.

[Article in Chinese]

Authors

B S Chen¹, G C Chen, J H Hsieh, M H Meng, T F Tsai, J J Liu, J H Huang, T H Chang

Affiliation

¹ Development Center for Biotechnology, Taipei, Taiwan, R.O.C.

PMID: 1697521

Abstract

Development of large-scale monoclonal antibody production using industrial scale hollow fiber bioreactor is described. Hybridoma cell lines H505AC and A306AA were cultivated in the hollow fiber perfusion bioreactor with a total fiber surface area of 7.2 m2 (6 x 1.2 m2) to produce anti-hepatitis B surface antigen (anti-HBsAg) monoclonal antibody IgM and anti-alpha-fetal protein (anti-AFP) monoclonal antibody IgG. The highest anti-HBsAg IgM productivity obtained during 49 days cultivation of H505AC was 0.135 g/day and the total accumulated IgM was 1.90 g. Results from the cultivation of A306AA in the hollow fiber module for 23 days showed the highest anti-AFP IgG productivity and the total accumulated IgG were 0.672 g/day and 7.21 g, respectively.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / biosynthesis*
Hepatitis B Surface Antigens / immunology*
Hydrogen-Ion Concentration
Immunoglobulin G / biosynthesis*
Immunoglobulin M / biosynthesis*
Methods
Mice
alpha-Fetoproteins / immunology*

Substances

Antibodies, Monoclonal
Hepatitis B Surface Antigens
Immunoglobulin G
Immunoglobulin M
alpha-Fetoproteins