A protein kinase signal-responsive gene carrier modified RGD peptide

Bioorg Med Chem Lett. 2006 Nov 15;16(22):5740-3. doi: 10.1016/j.bmcl.2006.08.096. Epub 2006 Sep 12.

Abstract

We have previously reported artificial gene-regulation systems responding to cyclic AMP-dependent protein kinase (PKA) using a cationic polymer. However, this polymer alone cannot deliver any gene into living cells. In the present work, we modified the signal-responsive polymer to the RGD peptide for the introduction of a polymer/DNA complex into living cells and succeeded in regulating the gene expression responding to intracellular PKA activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • DNA / genetics
  • DNA / metabolism
  • Enzyme Activation
  • Gene Expression Regulation / drug effects*
  • Genetic Therapy / methods*
  • HeLa Cells
  • Humans
  • Integrins / drug effects*
  • Integrins / genetics
  • Integrins / metabolism
  • Luciferases / genetics
  • Luciferases / metabolism
  • Oligopeptides / chemical synthesis
  • Oligopeptides / pharmacology*
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • Signal Transduction / drug effects*

Substances

  • Integrins
  • Oligopeptides
  • arginyl-glycyl-aspartic acid
  • DNA
  • Luciferases
  • Protein Kinases