A high-yield one-step purification method using copper-chelating chromatography for recombinant proteins fused with maltose-binding protein

Anal Biochem. 2006 Nov 1;358(1):152-4. doi: 10.1016/j.ab.2006.08.007. Epub 2006 Aug 22.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / isolation & purification*
  • Chelating Agents / chemistry*
  • Chromatography / methods*
  • Copper / chemistry*
  • Escherichia coli / metabolism
  • Humans
  • Maltose-Binding Proteins
  • Phosphoproteins / isolation & purification
  • Recombinant Fusion Proteins / isolation & purification*
  • Ribosomal Proteins / isolation & purification
  • Sepharose / chemistry

Substances

  • Carrier Proteins
  • Chelating Agents
  • Maltose-Binding Proteins
  • Phosphoproteins
  • Recombinant Fusion Proteins
  • Ribosomal Proteins
  • phosphoprotein P2, ribosomal
  • Copper
  • Sepharose