Direct nitric oxide measurement in human spermatozoa: flow cytometric analysis using the fluorescent probe, diaminofluorescein

Fanuel Lampiao; Hans Strijdom; Stefan S du Plessis

doi:10.1111/j.1365-2605.2006.00695.x

Direct nitric oxide measurement in human spermatozoa: flow cytometric analysis using the fluorescent probe, diaminofluorescein

Int J Androl. 2006 Oct;29(5):564-7. doi: 10.1111/j.1365-2605.2006.00695.x.

Authors

Fanuel Lampiao¹, Hans Strijdom, Stefan S du Plessis

Affiliation

¹ Department of Medical Physiology and Biochemistry, University of Stellenbosch, Tygerberg, South Africa.

PMID: 16968498
DOI: 10.1111/j.1365-2605.2006.00695.x

Abstract

We have demonstrated that intracellular nitric oxide (NO) can be detected and measured directly in human spermatozoa by flow cytometry using the fluorescent probe 4,5-diaminofluorescein-2/diacetate (10 microM). This method can measure both exogenously added as well as endogenously generated intracellular NO.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flow Cytometry / methods*
Fluorescein*
Fluorescent Dyes
Humans
Indicators and Reagents
Male
Nitric Oxide / analysis*
Spermatozoa / chemistry*

Substances

4,5-diaminofluorescein diacetate
Fluorescent Dyes
Indicators and Reagents
Nitric Oxide
Fluorescein