A chemical reporter strategy to probe glycoprotein fucosylation

J Am Chem Soc. 2006 Sep 20;128(37):12078-9. doi: 10.1021/ja064619y.

Abstract

Fucosylated glycoproteins are involved in many cell-cell recognition events and are markers of embryonic and malignant tissue. Here we report a method for rapid profiling of fucosylated glycoproteins from human cells using 6-azido fucose as a metabolic label.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Azides / chemistry
  • Azides / metabolism
  • Flow Cytometry
  • Fucose / analogs & derivatives*
  • Fucose / chemistry
  • Fucose / metabolism*
  • Glycoproteins / chemistry*
  • Glycoproteins / metabolism*
  • Humans
  • Jurkat Cells
  • Models, Molecular
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism

Substances

  • Azides
  • Glycoproteins
  • Polysaccharides
  • Fucose