Enzymatic synthesis of L-pipecolic acid by Delta1-piperideine-2-carboxylate reductase from Pseudomonas putida

Biosci Biotechnol Biochem. 2006 Sep;70(9):2296-8. doi: 10.1271/bbb.60125. Epub 2006 Sep 7.

Abstract

L-Pipecolic acid is a chiral pharmaceutical intermediate. An enzymatic system for the synthesis of L-pipecolic acid from L-lysine by commercial L-lysine alpha-oxidase from Trichoderma viride and an extract of recombinant Escherichia coli cells coexpressing Delta1-piperideine-2-carboxylate reductase from Pseudomonas putida and glucose dehydrogenase from Bacillus subtilis is described. A laboratory-scale process provided 27 g/l of L-pipecolic acid in 99.7% e.e.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / enzymology
  • Glucose 1-Dehydrogenase / metabolism
  • Industrial Microbiology / methods*
  • Mixed Function Oxygenases / metabolism
  • Oxidoreductases Acting on CH-NH Group Donors / metabolism*
  • Pipecolic Acids / metabolism*
  • Pseudomonas putida / enzymology
  • Pseudomonas putida / metabolism*
  • Trichoderma / enzymology

Substances

  • Pipecolic Acids
  • Mixed Function Oxygenases
  • Glucose 1-Dehydrogenase
  • lysine monooxygenase
  • Oxidoreductases Acting on CH-NH Group Donors
  • 1,2-didehydropipecolate reductase
  • pipecolic acid