Abstract
L-Pipecolic acid is a chiral pharmaceutical intermediate. An enzymatic system for the synthesis of L-pipecolic acid from L-lysine by commercial L-lysine alpha-oxidase from Trichoderma viride and an extract of recombinant Escherichia coli cells coexpressing Delta1-piperideine-2-carboxylate reductase from Pseudomonas putida and glucose dehydrogenase from Bacillus subtilis is described. A laboratory-scale process provided 27 g/l of L-pipecolic acid in 99.7% e.e.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / enzymology
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Glucose 1-Dehydrogenase / metabolism
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Industrial Microbiology / methods*
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Mixed Function Oxygenases / metabolism
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Oxidoreductases Acting on CH-NH Group Donors / metabolism*
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Pipecolic Acids / metabolism*
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Pseudomonas putida / enzymology
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Pseudomonas putida / metabolism*
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Trichoderma / enzymology
Substances
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Pipecolic Acids
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Mixed Function Oxygenases
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Glucose 1-Dehydrogenase
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lysine monooxygenase
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Oxidoreductases Acting on CH-NH Group Donors
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1,2-didehydropipecolate reductase
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pipecolic acid