Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae

Appl Environ Microbiol. 2006 Sep;72(9):6424-8. doi: 10.1128/AEM.02597-05.

Abstract

A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.

MeSH terms

  • Base Sequence
  • DNA Probes / genetics
  • DNA, Bacterial / genetics
  • Genes, Bacterial
  • Genes, Regulator
  • Humans
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • Sensitivity and Specificity
  • Vibrio cholerae / genetics*
  • Vibrio cholerae / isolation & purification
  • Vibrio cholerae / pathogenicity
  • Virulence / genetics
  • Water Microbiology

Substances

  • DNA Probes
  • DNA, Bacterial