Isolation and characterization of a new T-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157:H7 levels in sheep

Raul R Raya; Peter Varey; Rebecca A Oot; Michael R Dyen; Todd R Callaway; Tom S Edrington; Elizabeth M Kutter; Andrew D Brabban

doi:10.1128/AEM.03011-05

Isolation and characterization of a new T-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157:H7 levels in sheep

Appl Environ Microbiol. 2006 Sep;72(9):6405-10. doi: 10.1128/AEM.03011-05.

Authors

Raul R Raya¹, Peter Varey, Rebecca A Oot, Michael R Dyen, Todd R Callaway, Tom S Edrington, Elizabeth M Kutter, Andrew D Brabban

Affiliation

¹ The Evergreen State College, Olympia, WA 98505, USA.

Abstract

Bacteriophage CEV1 was isolated from sheep resistant to Escherichia coli O157:H7 colonization. In vitro, CEV1 efficiently infected E. coli O157:H7 grown both aerobically and anaerobically. In vivo, sheep receiving a single oral dose of CEV1 showed a 2-log-unit reduction in intestinal E. coli O157:H7 levels within 2 days compared to levels in the controls.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Administration, Oral
Aerobiosis
Anaerobiosis
Animals
Escherichia coli O157 / isolation & purification
Escherichia coli O157 / pathogenicity
Escherichia coli O157 / virology*
Female
Food Microbiology
Microscopy, Electron
Probiotics
Sheep / microbiology*
T-Phages / isolation & purification*
T-Phages / pathogenicity
T-Phages / ultrastructure

Abstract

Publication types

MeSH terms

Grants and funding