Diagnostic value of reverse transcription-PCR for detection of cytomegalovirus pp67 in samples from solid-organ transplant recipients

Ursula Meyer-Koenig; Ilona Romberg; Kerstin Schneider; Manfred Weidmann; Winfried V Kern; Frank T Hufert

doi:10.1128/JCM.00493-06

Diagnostic value of reverse transcription-PCR for detection of cytomegalovirus pp67 in samples from solid-organ transplant recipients

J Clin Microbiol. 2006 Sep;44(9):3394-6. doi: 10.1128/JCM.00493-06.

Authors

Ursula Meyer-Koenig¹, Ilona Romberg, Kerstin Schneider, Manfred Weidmann, Winfried V Kern, Frank T Hufert

Affiliation

¹ Department of Virology, Institute for Medical Microbiology, University Hospital Freiburg, Hermann-Herder-Str. 11, D 79104 Freiburg, Germany. ursula.meyer-koenig@uniklinik-freiburg.de

Abstract

We evaluated a highly sensitive quantitative real-time one-step reverse transcription-PCR (RT-PCR) for detection of human cytomegalovirus pp67 transcripts in monitoring of solid-organ transplant recipients. Results were compared with those of pp65 antigen testing and quantitative DNA-PCR. Due to a low clinical sensitivity, the pp67 RT-PCR was not able to replace these assays.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Cytomegalovirus / genetics
Cytomegalovirus / isolation & purification
Cytomegalovirus Infections / diagnosis*
Cytomegalovirus Infections / virology
DNA, Viral / analysis
Humans
Organ Transplantation / adverse effects*
Phosphoproteins* / blood
Polymerase Chain Reaction
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Viral / genetics
RNA, Viral / metabolism
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Viral Matrix Proteins* / blood

Substances

DNA, Viral
Phosphoproteins
RNA, Messenger
RNA, Viral
Viral Matrix Proteins
cytomegalovirus matrix protein 65kDa
pp67 protein, human cytomegalovirus