Abstract
The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport subunits and for hypothetical and unknown-function proteins.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / genetics
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Alkaline Phosphatase / metabolism*
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Base Sequence*
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Biological Transport
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Corynebacterium pseudotuberculosis / genetics*
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Corynebacterium pseudotuberculosis / growth & development
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Corynebacterium pseudotuberculosis / metabolism
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DNA Transposable Elements*
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Enterococcus faecalis / enzymology
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Enterococcus faecalis / genetics
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Molecular Sequence Data
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Mutagenesis, Insertional / methods*
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Sequence Analysis, DNA
Substances
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Bacterial Proteins
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DNA Transposable Elements
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Alkaline Phosphatase
Associated data
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GENBANK/CZ171041
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GENBANK/CZ171042
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GENBANK/CZ171043
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GENBANK/CZ171044
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GENBANK/CZ171045
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GENBANK/CZ171046
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GENBANK/CZ171047
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GENBANK/CZ171048
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GENBANK/CZ171049
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GENBANK/CZ171050
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GENBANK/CZ171051
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GENBANK/CZ171052
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GENBANK/CZ171053
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GENBANK/CZ171054
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GENBANK/CZ171055
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GENBANK/CZ171056
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GENBANK/CZ171057
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GENBANK/CZ171058
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GENBANK/CZ171059
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GENBANK/CZ171060
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GENBANK/CZ171061
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GENBANK/CZ171062
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GENBANK/CZ171063
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GENBANK/CZ171064
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GENBANK/CZ171065
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GENBANK/CZ171066
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GENBANK/CZ171067
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GENBANK/CZ171068
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GENBANK/CZ171069
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GENBANK/CZ171070
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GENBANK/CZ171071
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GENBANK/CZ171072
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GENBANK/CZ171073
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GENBANK/CZ171074