Generation of a reporter cell line for detection of infectious varicella-zoster virus and its application to antiviral studies

Guan-Qing Wang; Tatsuo Suzutani; Yumiko Yamamoto; Yoshiko Fukui; Naoki Nozawa; D Scott Schmid; Ichiro Kurane; Naoki Inoue

doi:10.1128/AAC.00342-06

Generation of a reporter cell line for detection of infectious varicella-zoster virus and its application to antiviral studies

Antimicrob Agents Chemother. 2006 Sep;50(9):3142-5. doi: 10.1128/AAC.00342-06.

Authors

Guan-Qing Wang¹, Tatsuo Suzutani, Yumiko Yamamoto, Yoshiko Fukui, Naoki Nozawa, D Scott Schmid, Ichiro Kurane, Naoki Inoue

Affiliation

¹ Department of Virology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.

Abstract

To simplify the titration of infectious varicella-zoster virus (VZV), we generated a reporter cell line that produced luciferase in a dose-dependent manner upon infection with cell-free VZV. A few VZV-infected cells were detectable by coculturing with the cell line. We demonstrated the usefulness of the cell line for antiviral studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antiviral Agents / pharmacology*
Base Sequence
Cell Line / enzymology*
Cell Line / virology*
Genes, Reporter / genetics
Herpesvirus 3, Human / drug effects
Herpesvirus 3, Human / genetics*
Luciferases / biosynthesis
Luciferases / genetics
Microbial Sensitivity Tests / methods*
Molecular Sequence Data
Open Reading Frames
Promoter Regions, Genetic

Substances

Antiviral Agents
Luciferases