Abstract
By systematic investigation, glutamate and a mixture of metal ions were identified as factors limiting the production of nattokinase in Bacillus subtilis. Consequently, in medium supplemented with these materials, the recombinant strain secreted 4 times more nattokinase (260 mg l(-1)) than when grown in the unsupplemented medium.
MeSH terms
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Bacillus subtilis / genetics*
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Bacillus subtilis / metabolism
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Cells, Cultured
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Genetic Vectors
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Models, Biological
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Plasmids
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Subtilisins / biosynthesis*
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Subtilisins / genetics
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Subtilisins / metabolism
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Up-Regulation
Substances
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Recombinant Proteins
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Subtilisins
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nattokinase