Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp. OU01

Yuying Sun; Baoqin Han; Wanshun Liu; Jiquan Zhang; Xingshuang Gao

doi:10.1016/j.biortech.2006.07.020

Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp. OU01

Bioresour Technol. 2007 May;98(8):1548-53. doi: 10.1016/j.biortech.2006.07.020. Epub 2006 Aug 22.

Authors

Yuying Sun¹, Baoqin Han, Wanshun Liu, Jiquan Zhang, Xingshuang Gao

Affiliation

¹ College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.

PMID: 16930999
DOI: 10.1016/j.biortech.2006.07.020

Abstract

The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacterium sp. OU01 in shake-flask. A significant influence of (NH(4))(2)SO(4), MgSO(4).7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0g/L (NH(4))(2)SO(4), 1.3g/L MgSO(4) and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6U/mL to 118U/mL.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Actinomycetales / enzymology*
Bioreactors*
Culture Media / metabolism*
Glycoside Hydrolases / biosynthesis*
Hydrogen-Ion Concentration

Substances

Culture Media
Glycoside Hydrolases
chitosanase