A nanophosphor-based method for selective DNA recovery in Synthosomes

Biotechnol J. 2006 Jul-Aug;1(7-8):828-34. doi: 10.1002/biot.200600042.

Abstract

A nanocompartment system composed of an ABA triblock copolymer, where A is poly(dimethylsiloxane) and B is poly(2-methyloxazoline), has been developed for selective recovery and detection of DNA. Translocation of TAMRA-labeled complementary primers into the nanocompartment system has been achieved through two deletion mutants (FhuA Delta1-129; FhuA Delta1-160) of the channel protein FhuA. Translocation was monitored by fluorescence resonance energy transfer through hybridization of the TAMRA-labeled primer to the complementary sequence of a nanophosphor-DNA-conjugate, which reduces its half-life (FhuA Delta1-129, 16.0% reduced; FhuA Delta1-160, 39.0% reduced).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocompatible Materials / chemistry*
  • DNA / isolation & purification*
  • Liposomes / chemistry*
  • Luminescent Measurements / methods*
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure
  • Particle Size
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / methods*

Substances

  • Biocompatible Materials
  • Liposomes
  • DNA