Spectrophotometric method for exploring 3-methyl-2-butene-1-thiol (MBT) formation in lager

J Agric Food Chem. 2006 Aug 23;54(17):6123-9. doi: 10.1021/jf060515v.

Abstract

The disappearance of riboflavin absorbance at 445 nm from beers or model beers on light exposure is directly linked to light-struck character formation. The addition of (+)-catechin, (-)-epicatechin, tryptophol, or ascorbic acid was able to reduce, but not stop, absorbance loss or light-struck character formation in either model beer or mainstream lager that was exposed to light. When isohumulone was present in model beer, the inhibitory effect of (+)-catechin, (-)-epicatechin, or tryptophol decreased with increasing isohumulone. The spectrophotometric method used in this study is a simple and effective method for determining light-struck susceptibility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascorbic Acid / pharmacology
  • Beer / analysis*
  • Butanes / chemistry*
  • Catechin / pharmacology
  • Cyclopentanes / analysis
  • Indoles / pharmacology
  • Light
  • Photochemistry
  • Riboflavin / analysis
  • Spectrophotometry / methods*
  • Sulfhydryl Compounds / chemistry*

Substances

  • Butanes
  • Cyclopentanes
  • Indoles
  • Sulfhydryl Compounds
  • tryptophol
  • Catechin
  • isohumulone
  • 3-methyl-2-butene-1-thiol
  • Ascorbic Acid
  • Riboflavin