Abstract
In plants and animals, small peptide ligands that signal in cell-cell communication have been suggested to be a crucial component of development. A bioassay of single-cell transdifferentation demonstrates that a dodecapeptide with two hydroxyproline residues is the functional product of genes from the CLE family, which includes CLAVATA3 in Arabidopsis. The dodecapeptide suppresses xylem cell development at a concentration of 10(-11) M and promotes cell division. An application, corresponding to all 26 Arabidopsis CLE protein family members, of synthetic dodecapeptides reveals two counteracting signaling pathways involved in stem cell fate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / cytology
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / metabolism
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Asteraceae / cytology*
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Base Sequence
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Biological Assay
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Cell Communication
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Cell Differentiation*
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Cells, Cultured
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Ligands
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Meristem / cytology
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Molecular Sequence Data
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Oligopeptides / chemistry
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Oligopeptides / isolation & purification
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Oligopeptides / metabolism*
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Oligopeptides / pharmacology
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Plant Proteins / chemistry
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Plant Proteins / metabolism*
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Plant Roots / cytology
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Plant Roots / growth & development
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Plant Structures / cytology*
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Signal Transduction*
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Stem Cells / cytology*
Substances
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AT2G27250 protein, Arabidopsis
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Arabidopsis Proteins
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Ligands
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Oligopeptides
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Plant Proteins