The mobilization of fatty acids (FAs) during food deprivation is a selective process in laboratory rodents and humans. The site-specific differences in adipose tissue functions - e.g. energy storage versus insulation - should also affect the use of different FAs. To study this, 16 female minks were randomly assigned into the control group or fasted for 5 days. Preferential mobilization of n-3 polyunsaturated FAs (PUFAs) during fasting caused a decrease in the n-3/n-6 PUFA ratio in fat and liver. In addition, the minks utilized short-chain FAs efficiently in all fat depots, but long-chain FAs - 20:0, 20:1n-11, 20:1n-9, 22:1n-11 and 24:1n-9 - were preserved. The number of double bonds in the FA chain correlated positively with mobilization rate in the retroperitoneal fat. The observed negative correlation between mobilization rate and the location of the first double bond from the methyl end may be due to peroxisomal chain-shortening of long-chain FAs and not the double bond position per se. As a result, minks are able to preserve a low melting point and fluidity of the subcutaneous fat depots, which would be essential to a Northern semi-aquatic mammal.