Short-sequence-repeat analysis of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium isolates collected from animals throughout the United States reveals both stability of loci and extensive diversity

N Beth Harris; Janet B Payeur; Vivek Kapur; Srinand Sreevatsan

doi:10.1128/JCM.00584-06

Short-sequence-repeat analysis of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium isolates collected from animals throughout the United States reveals both stability of loci and extensive diversity

J Clin Microbiol. 2006 Aug;44(8):2970-3. doi: 10.1128/JCM.00584-06.

Authors

N Beth Harris¹, Janet B Payeur, Vivek Kapur, Srinand Sreevatsan

Affiliation

¹ National Veterinary Services Laboratories, Ames, IA 50010, USA.

Abstract

We analyzed the multilocus short sequence repeats (SSRs) of 211 and 56 isolates of Mycobacterium avium subsp. paratuberculosis and M. avium subsp. avium, respectively. The M. avium subsp. paratuberculosis isolates could be differentiated into 61 genotypes. The M. avium subsp. avium isolates showed limited diversity. These SSRs are stable and suitable for studying the molecular epidemiology of M. avium subsp. paratuberculosis.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bacterial Typing Techniques*
Cattle
Cattle Diseases / microbiology
DNA, Bacterial / genetics
Genotype
Mycobacterium avium / classification*
Mycobacterium avium / genetics
Mycobacterium avium / isolation & purification
Mycobacterium avium subsp. paratuberculosis / classification*
Mycobacterium avium subsp. paratuberculosis / genetics
Mycobacterium avium subsp. paratuberculosis / isolation & purification
Paratuberculosis / microbiology*
Repetitive Sequences, Nucleic Acid*
Tuberculosis / microbiology
Tuberculosis / veterinary*
United States

Substances

DNA, Bacterial