The effect of ultrasound stimulation versus bioreactors on neocartilage formation in tissue engineering scaffolds seeded with human chondrocytes in vitro

Shan-hui Hsu; Chi-Ching Kuo; Shu Wen Whu; Chen-Huan Lin; Ching-Lin Tsai

doi:10.1016/j.bioeng.2006.05.029

The effect of ultrasound stimulation versus bioreactors on neocartilage formation in tissue engineering scaffolds seeded with human chondrocytes in vitro

Biomol Eng. 2006 Oct;23(5):259-64. doi: 10.1016/j.bioeng.2006.05.029. Epub 2006 Jun 29.

Authors

Shan-hui Hsu¹, Chi-Ching Kuo, Shu Wen Whu, Chen-Huan Lin, Ching-Lin Tsai

Affiliation

¹ Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan, ROC. shhsu@nchu.edu.tw <shhsu@nchu.edu.tw>

PMID: 16890016
DOI: 10.1016/j.bioeng.2006.05.029

Abstract

Pulsed ultrasound (1 MHz, 67 mW/cm(2) Ispta, and 10 min/day) promoted cell proliferation and matrix deposition in low-density 2D ( approximately 6 x 10(3)cells/cm(2)) as well as 3D ( approximately 4 x 10(6)cells/cm(3)) chondrocyte cultures. The beneficial effect of ultrasound on neocartilage formation only last 28 days, shorter than that of bioreactors.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Bioreactors*
Cartilage, Articular / cytology
Cartilage, Articular / growth & development*
Cartilage, Articular / radiation effects
Cell Culture Techniques / methods
Cell Differentiation / radiation effects
Cell Proliferation / radiation effects
Cells, Cultured
Chondrocytes / cytology*
Chondrocytes / physiology*
Chondrocytes / radiation effects
Chondrogenesis / drug effects
Chondrogenesis / physiology
Humans
Mechanotransduction, Cellular / physiology*
Mechanotransduction, Cellular / radiation effects
Physical Stimulation / methods
Sonication*
Tissue Engineering / methods*