We investigated the effects of intracerebroventricular (ICV) and intraperitoneal (IP) administration of pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) on food intake in the goldfish, Carassius auratus. Cumulative food intake was significantly decreased by ICV injection of PACAP or VIP. Similarly, IP administration of PACAP or VIP induced a significant decrease in food intake. ICV injection of PACAP or VIP also induced a significant decrease in locomotor activity. These results suggest that PACAP and VIP may be involved in the regulation of feeding in goldfish.