[Suggestions concerning serological diagnosis of echinococcosis in Poland]

Wiad Parazytol. 1999;45(2):229-33.
[Article in Polish]

Abstract

It has been suggested to change the present way of serological diagnosis of echinococcosis in Poland which utilizes immunoelectrophoresis for confirmation of positive results in a screening test. Diagnostic procedure should be indicated with a sensitive test, such as indirect haemagglutination or immunoenzymatic assay, and every positive result should be verifies later using a Western-blot method in order to detect the most specific reaction with 8 kDa fraction of Echinococcus antigen. Further diferentiation of the infecting species may be accomplished with the aid of Em2 plus ELISA test which detects antibodies specific for E. multilocularis.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Antigens, Helminth / analysis*
  • Biomarkers / analysis
  • Blotting, Western / methods
  • Echinococcosis, Hepatic / diagnosis*
  • Echinococcus / classification
  • Echinococcus / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Poland
  • Sensitivity and Specificity
  • Serologic Tests

Substances

  • Antigens, Helminth
  • Biomarkers