Abstract
Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8 degrees C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche.
MeSH terms
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Animals
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Cattle
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Colony Count, Microbial
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Electrophoresis, Gel, Pulsed-Field / methods*
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Food Handling / methods
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Food Packaging / methods*
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Hydrogen-Ion Concentration
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Lactobacillus / classification
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Lactobacillus / genetics
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Lactobacillus / isolation & purification*
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Leuconostoc / classification
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Leuconostoc / genetics
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Leuconostoc / isolation & purification*
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Meat / microbiology*
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Molecular Sequence Data
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Polymerase Chain Reaction / methods*
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Sequence Analysis, DNA
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Species Specificity
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Temperature
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Time Factors
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Vacuum
Associated data
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GENBANK/AY946201
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GENBANK/DQ336384
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GENBANK/DQ336385