Abstract
Yu-Ping-Feng-Powder (YP), a traditional Chinese medicine prescription, is widely applied in China for the cure and prevention of diseases related to immunodeficiency. To test whether the fractioned polysaccharides (YPF-P) isolated from YP have immunomodulating activities, the effects of YPF-P on cyclophosphamide (Cy)-treated mice were studied in relation to phagocytosis of macrophage, splenocyte proliferation, and humoral, and cellular immunity parameters. It was found that YPF-P enhances phagocytic activity, augments ConA- or LPS-stimulated T cell proliferation, increases the quantitative haemolysis of SRBC (QHS) and delayed-type hypersensitivity reaction (DTH) to dinitrofluorobenzene. Hence, YPF-P restored the immuno-competence suppressed by Cy. YPF-P also augmented IL-2 and IFN-gamma production, but failed to increase IL-4 production, which indicates that there is high probability that it enhance Th1 function. These results suggested that YPF-P has immunomodulating effects and that the polysaccharides constitute one of the active components of YP.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Animals
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Antibody Formation / drug effects
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Cell Proliferation / drug effects
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Cells, Cultured
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Cyclophosphamide / administration & dosage
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Cyclophosphamide / toxicity*
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Drugs, Chinese Herbal / chemistry*
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Immunity, Cellular / drug effects
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Immunologic Factors / administration & dosage
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Immunologic Factors / pharmacology*
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Immunosuppressive Agents / administration & dosage
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Immunosuppressive Agents / toxicity
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Injections, Intraperitoneal
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Interferon-gamma / biosynthesis
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Interleukin-2 / biosynthesis
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Interleukin-4 / biosynthesis
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / immunology
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Mice
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Mice, Inbred BALB C
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Mitogens / pharmacology
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Phagocytosis / drug effects
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Polysaccharides / administration & dosage
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Polysaccharides / isolation & purification
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Polysaccharides / pharmacology*
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Powders
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Spleen / cytology
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Spleen / drug effects
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Spleen / metabolism
Substances
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Drugs, Chinese Herbal
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Immunologic Factors
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Immunosuppressive Agents
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Interleukin-2
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Mitogens
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Polysaccharides
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Powders
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Interleukin-4
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Interferon-gamma
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Cyclophosphamide