Determination of coenzyme Q10 in human breast milk by high-performance liquid chromatography

Biomed Chromatogr. 2006 Dec;20(12):1336-43. doi: 10.1002/bmc.702.

Abstract

An isocratic HPLC method was developed for the determination of coenzyme Q(10) (CoQ(10)) in human breast milk. After a single-step liquid-liquid extraction, the milk extract was injected directly into the HPLC system. The analytical method is based on pre-column inline treatment of CoQ(10). Chromatographic separation of CoQ(10) and coenzyme Q(9) (CoQ(9)) internal standard was achieved using a reversed-phase Microsorb-MV C(18) analytical column. CoQ(10) and CoQ(9) were monitored by an electrochemical detector (ECD). An excellent linearity (r = 0.999) was observed for CoQ(10) in the concentration range 0.06-2.5 micromol L(-1) in breast milk. The limit of quantitation (LOQ) was 60 nmol L(-1). Coefficients of variations (CVs) for intra-day and inter-day assay precisions were less than 5%. A total of 194 breast milk samples were analyzed for the CoQ(10) concentration; the mean value was 0.32 +/- 0.21 micromol L(-1).

Publication types

  • Research Support, N.I.H., Extramural
  • Validation Study

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Coenzymes
  • Drug Stability
  • Female
  • Humans
  • Milk, Human / chemistry*
  • Ubiquinone / analogs & derivatives*
  • Ubiquinone / analysis

Substances

  • Coenzymes
  • Ubiquinone
  • coenzyme Q10