Methodology of reversible protein labeling for ratiometric fluorescent measurement

Nobuaki Soh; Daisuke Seto; Koji Nakano; Toshihiko Imato

doi:10.1039/b515777c

Methodology of reversible protein labeling for ratiometric fluorescent measurement

Mol Biosyst. 2006 Feb;2(2):128-31. doi: 10.1039/b515777c. Epub 2005 Dec 16.

Authors

Nobuaki Soh¹, Daisuke Seto, Koji Nakano, Toshihiko Imato

Affiliation

¹ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.

PMID: 16880930
DOI: 10.1039/b515777c

Abstract

The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag.

MeSH terms

Amino Acid Sequence
Amino Acids / chemistry
Buffers
Fluorescent Dyes / analysis*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Models, Biological
Proteins / chemistry
Proteins / metabolism*
Spectrometry, Fluorescence

Substances

Amino Acids
Buffers
Fluorescent Dyes
Proteins