Characterization of the CHORI-240 BAC clones containing the bovine CSN1S1, CSN2, STATH, CSN1S2 and CSN3 genes

Alexei A Sazanov; Tadeusz Malewski; Stanisław Kamiński; Lech Zwierzchowski

doi:10.1007/BF03194630

Characterization of the CHORI-240 BAC clones containing the bovine CSN1S1, CSN2, STATH, CSN1S2 and CSN3 genes

J Appl Genet. 2006;47(3):243-5. doi: 10.1007/BF03194630.

Authors

Alexei A Sazanov¹, Tadeusz Malewski, Stanisław Kamiński, Lech Zwierzchowski

Affiliation

¹ Laboratory of Molecular Genome Organization, Institute of Farm Animal Genetics and Breeding, Russian Academy of Agricultural Sciences, Moskovskoye sh. St Petersburg-Pushkin, Russia.

PMID: 16877803
DOI: 10.1007/BF03194630

Abstract

Nineteen BAC clones were identified by hybridization of the bovine genomic BAC library CHORI-240 with mixed CSN1S1- and CSN3-specific probes. Two of the clones were shown to contain the genes CSN1S1, CSN1S2, CSN2, STATH and CSN3, and five were proved to include the genes CSN2, STATH, CSN1S2 and CSN3. These data showed that the BAC contig was established for the whole casein cluster, including all known five genes.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Caseins / genetics*
Cattle / genetics*
Chromosomes, Artificial, Bacterial*
DNA Primers
Molecular Sequence Data
Nucleic Acid Hybridization / methods
Sequence Analysis, DNA

Substances

Caseins
DNA Primers