Abstract
Impaired proteasome function is a potential mechanism for dopaminergic neuron degeneration. To model this molecular defect, we administered systemically the reversible lipophilic proteasome inhibitor, carbobenzoxy-L-isoleucyl-gamma-t-butyl-L-glutamyl-L-alanyl-L-leucinal (PSI), to rodents. In contrast to a previous report, this approach failed to cause any detectable behavioral or neuropathological abnormality in either rats or mice. Although theoretically appealing, this specific model of Parkinson's disease appears to exhibit poor reproducibility.
Publication types
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Comment
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cell Line
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Cysteine Proteinase Inhibitors / toxicity*
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Disease Models, Animal
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Flow Cytometry
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Green Fluorescent Proteins / genetics
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Humans
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Immunohistochemistry
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Neurons / drug effects
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Neurons / metabolism
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Oligopeptides / toxicity*
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Parkinson Disease, Secondary / chemically induced*
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Rats
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Rats, Sprague-Dawley
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Tyrosine 3-Monooxygenase / metabolism
Substances
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Cysteine Proteinase Inhibitors
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Oligopeptides
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benzyloxycarbonyl-isoleucyl-glutamyl(O-tert-butyl)-alanyl-leucinal
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Green Fluorescent Proteins
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Tyrosine 3-Monooxygenase