A unique and universal molecular barcode array

Sarah E Pierce; Eula L Fung; Daniel F Jaramillo; Angela M Chu; Ronald W Davis; Corey Nislow; Guri Giaever

doi:10.1038/nmeth905

A unique and universal molecular barcode array

Nat Methods. 2006 Aug;3(8):601-3. doi: 10.1038/nmeth905.

Authors

Sarah E Pierce¹, Eula L Fung, Daniel F Jaramillo, Angela M Chu, Ronald W Davis, Corey Nislow, Guri Giaever

Affiliation

¹ Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA.

PMID: 16862133
DOI: 10.1038/nmeth905

Abstract

Molecular barcode arrays allow the analysis of thousands of biological samples in parallel through the use of unique 20-base-pair (bp) DNA tags. Here we present a new barcode array, which is unique among microarrays in that it includes at least five replicates of every tag feature. The use of smaller dispersed replicate features dramatically improves performance versus a single larger feature and allows the correction of previously undetectable hybridization defects.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Base Sequence
DNA Probes / genetics*
Equipment Design
Equipment Failure Analysis
Expressed Sequence Tags*
In Situ Hybridization, Fluorescence / instrumentation*
In Situ Hybridization, Fluorescence / methods
Molecular Probe Techniques / instrumentation*
Molecular Sequence Data
Reproducibility of Results
Sensitivity and Specificity
Sequence Alignment / instrumentation*
Sequence Alignment / methods
Sequence Analysis, DNA / instrumentation*
Sequence Analysis, DNA / methods

Substances

DNA Probes