Random amplified polymorphic DNA analysis of genetically modified organisms

J Biotechnol. 2006 Dec 15;127(1):161-6. doi: 10.1016/j.jbiotec.2006.06.001. Epub 2006 Jun 12.

Abstract

Randomly amplified polymorphic DNA (RAPD) was used to analyzed 78 samples comprises of certified reference materials (soya and maize powder), raw seeds (soybean and maize), processed food and animal feed. Combination assay of two arbitrary primers in the RAPD analysis enable to distinguish genetically modified organism (GMO) reference materials from the samples tested. Dendrogram analysis revealed 13 clusters at 45% similarity from the RAPD. RAPD analysis showed that the maize and soybean samples were clustered differently besides the GMO and non-GMO products.

MeSH terms

  • Food Analysis / methods
  • Food, Genetically Modified*
  • Glycine max / genetics*
  • Plants, Genetically Modified / genetics*
  • Polymorphism, Genetic
  • Random Amplified Polymorphic DNA Technique*
  • Reference Values
  • Zea mays / genetics*