Abstract
A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA-D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n-3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1.
MeSH terms
-
Amino Acid Sequence
-
Bacterial Proteins / chemistry
-
Bacterial Proteins / genetics*
-
Bacterial Proteins / metabolism
-
Base Sequence
-
Cloning, Molecular
-
Escherichia coli / genetics
-
Fatty Acids, Omega-3 / biosynthesis*
-
Genes, Bacterial*
-
Molecular Sequence Data
-
Moritella / enzymology*
-
Moritella / genetics
-
Polymerase Chain Reaction
-
Protein Structure, Tertiary
-
Sequence Alignment
-
Transferases (Other Substituted Phosphate Groups) / chemistry
-
Transferases (Other Substituted Phosphate Groups) / genetics*
-
Transferases (Other Substituted Phosphate Groups) / metabolism
Substances
-
Bacterial Proteins
-
Fatty Acids, Omega-3
-
phosphopantetheinyl transferase
-
Transferases (Other Substituted Phosphate Groups)