Abstract
Bioassay-directed fractionation of an extract of the root and bark of Podocarpus madagascariensis resulted in the isolation of a new totarol diterpenoid (1) in addition to the three known cytotoxic diterpenoids 19-hydroxytotarol (2), totaradiol (3), and 4beta-carboxy-19-nor-totarol (4). The structure of the new compound 1 was established as methyl-13-hydroxy-14-isopropyl-9(11),12,14(8)-podocarpatriene-19-oate on the basis of 1D and 2D NMR spectroscopic interpretation and methylation of 4. All the compounds exhibited cytotoxic activity against the A2780 ovarian cancer cell line.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology*
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Cell Line, Tumor
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Cycadopsida / chemistry*
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Diterpenes / chemistry
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Diterpenes / isolation & purification*
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Diterpenes / pharmacology*
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Ecosystem
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Female
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Humans
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Madagascar
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Nuclear Magnetic Resonance, Biomolecular
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Ovarian Neoplasms / drug therapy
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Plant Bark / chemistry
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Plant Roots / chemistry
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Spectrometry, Mass, Fast Atom Bombardment
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
Substances
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Antineoplastic Agents, Phytogenic
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Diterpenes