Abstract
An unidentified Gram-negative-staining, aerobic, rod-shaped, spore-forming bacterium was isolated from a sample of cerebrospinal fluid. Based on comparative analysis of 16S rRNA gene sequences and phenotypic characteristics, the novel isolate was included in the Bacillus sphaericus-like group. The isolate was closely related to Bacillus odysseyi and Bacillus silvestris, with 96.2 and 94.4 % 16S rRNA gene sequence similarity, respectively. The major fatty acid was iso-C(15 : 0) (48 %). The name Bacillus massiliensis sp. nov. is proposed for the novel isolate, with strain 4400831(T) (=CIP 108446(T)=CCUG 49529(T)) as the type strain.
MeSH terms
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Bacillaceae Infections / cerebrospinal fluid
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Bacillaceae Infections / microbiology*
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Bacillus / chemistry
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Bacillus / classification*
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Bacillus / cytology
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Bacillus / genetics
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Bacillus / isolation & purification*
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Bacillus / physiology
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Bacterial Typing Techniques
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Cerebrospinal Fluid / microbiology*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Fatty Acids / chemistry
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Fatty Acids / isolation & purification
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Genes, rRNA
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Gentian Violet
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Humans
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Male
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Middle Aged
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Molecular Sequence Data
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Phenazines
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Phylogeny
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
Substances
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DNA, Bacterial
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DNA, Ribosomal
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Fatty Acids
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Gram's stain
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Phenazines
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RNA, Bacterial
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RNA, Ribosomal, 16S
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Gentian Violet