Abstract
A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin (rpoB) and high-level isoniazid (katG) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.
MeSH terms
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Antibiotics, Antitubercular / pharmacology
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Antitubercular Agents / pharmacology*
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Body Fluids / microbiology
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Drug Resistance, Bacterial / genetics*
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Genotype
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Humans
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Isoniazid / pharmacology*
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Microbial Sensitivity Tests / methods*
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Mycobacterium tuberculosis / drug effects*
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Mycobacterium tuberculosis / genetics
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Polymerase Chain Reaction / methods
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Rifampin / pharmacology*
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Tuberculosis, Multidrug-Resistant / microbiology
Substances
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Antibiotics, Antitubercular
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Antitubercular Agents
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Isoniazid
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Rifampin