Crystallization and preliminary X-ray diffraction analysis of a protease inhibitor from the haemolymph of the Indian tasar silkworm Antheraea mylitta

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt 7):669-71. doi: 10.1107/S1744309106020719. Epub 2006 Jun 10.

Abstract

A protein with inhibitory activity against fungal proteases was purified from the haemolymph of the Indian tasar silkworm Antheraea mylitta and was crystallized using the hanging-drop vapour-diffusion method. Polyethylene glycol 3350 was used as a precipitant. Crystals belonged to space group P6(3)22, with unit-cell parameters a = b = 60.6, c = 85.1 angstroms. X-ray diffraction data were collected and processed to a maximum resolution of 2.1 angstroms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bombyx / enzymology*
  • Crystallization
  • Fungal Proteins / antagonists & inhibitors
  • Hemolymph / enzymology*
  • Protease Inhibitors / chemistry*
  • Protease Inhibitors / isolation & purification
  • Protease Inhibitors / pharmacology*
  • X-Ray Diffraction

Substances

  • Fungal Proteins
  • Protease Inhibitors