Abstract
Berkeley Pit Lake is an abandoned open-pit copper mine filled with 30 billion gallons of acidic, metal-contaminated water. This harsh environment is proving to be a source of unusual microorganisms that produce novel bioactive metabolites. Bioassay-guided fractionation using signal transduction enzyme assays led to the isolation of the novel spiroketal, berkelic acid 1, and of the known gamma-pyrone, spiciferone A 4. Berkelic acid has shown selective, nanomolar activity against OVCAR-3, an ovarian cancer cell line in the National Cancer Institute cell line screen. The isolation and characterization of these compounds are reported here.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology*
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Cell Line, Tumor
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Drug Screening Assays, Antitumor
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Heterocyclic Compounds, 2-Ring / chemistry
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Humans
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Magnetic Resonance Spectroscopy / methods
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Magnetic Resonance Spectroscopy / standards
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Molecular Conformation
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Penicillium / chemistry*
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Reference Standards
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Sensitivity and Specificity
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Spiro Compounds / chemistry*
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Spiro Compounds / isolation & purification
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Spiro Compounds / pharmacology*
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Waste Products
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Water / chemistry
Substances
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Antineoplastic Agents
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Heterocyclic Compounds, 2-Ring
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Spiro Compounds
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Waste Products
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berkelic acid
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spiciferone A
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Water