Differential expression of mouse orexin receptor type-2 (OX2R) variants in the mouse brain

Jing Chen; Emmanouil Karteris; Dawn Collins; Harpal S Randeva

doi:10.1016/j.brainres.2006.05.054

Differential expression of mouse orexin receptor type-2 (OX2R) variants in the mouse brain

Brain Res. 2006 Aug 4;1103(1):20-4. doi: 10.1016/j.brainres.2006.05.054. Epub 2006 Jun 27.

Authors

Jing Chen¹, Emmanouil Karteris, Dawn Collins, Harpal S Randeva

Affiliation

¹ Endocrinology and Metabolism Group, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK.

PMID: 16806113
DOI: 10.1016/j.brainres.2006.05.054

Abstract

We have identified two novel mouse orexin type-2 receptor (OX2R) splice variants: OX2alphaR and OX2betaR. Here, we have mapped in detail the differential gene expression of OX2alphaR and OX2betaR in the mouse brain. Using RT-PCR and in situ hybridization, we show that OX2alphaR is expressed in various areas of the mouse brain and has low expression in caudal part of cerebellum. OX2betaR is expressed in detectable levels, with the exception of striatum, midbrain and anterior part of cerebellum/pons. Our novel findings demonstrate the differential expression of OX2R splice variants in the mouse brain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Surface / biosynthesis*
Antigens, Surface / genetics*
Brain Chemistry / genetics*
Genetic Variation / physiology
In Situ Hybridization, Fluorescence
Mice
Orexin Receptors
Receptors, Cell Surface / biosynthesis*
Receptors, Cell Surface / genetics*
Reverse Transcriptase Polymerase Chain Reaction

Substances

Antigens, Surface
Cd200r1 protein, mouse
Orexin Receptors
Receptors, Cell Surface