Abstract
[reaction: see text] Phosphatidylinositol-3-phosphate (PtdIns(3)P) is a spatial regulator of vesicular trafficking and other vital cellular processes. We describe the asymmetric total synthesis of a metabolically stabilized analogue, phosphatidylinositol-3-methylenephosphate (PtdIns(3)MP) from a differentially protected myo-inositol. NMR studies of PtdIns(3)MP bound to the (15)N-labeled FYVE domain showed significant (1)H and (15)N chemical shift changes relative to the unliganded protein.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Inositol / chemistry
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Phosphatidylinositol Phosphates / chemical synthesis*
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Phosphatidylinositol Phosphates / chemistry
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Phosphatidylinositol Phosphates / metabolism
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Vesicular Transport Proteins / physiology*
Substances
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Phosphatidylinositol Phosphates
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Vesicular Transport Proteins
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phosphatidylinositol 3-phosphate
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phosphatidylinositol-3-methylenephosphate
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Inositol