Synthesis and single enzyme activity of a clicked lipase-BSA hetero-dimer

Chem Commun (Camb). 2006 May 21:(19):2012-4. doi: 10.1039/b516551b. Epub 2006 Feb 9.

Abstract

Click chemistry is used to construct a novel lipase-BSA hetero-dimer, in which the latter protein acts as a foot enabling the anchoring of the enzyme onto the surface for single enzyme studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascomycota / enzymology
  • Cattle
  • Clinical Enzyme Tests
  • Dimerization
  • Enzymes, Immobilized / chemistry
  • Lipase / chemistry
  • Lipase / genetics
  • Lipase / metabolism*
  • Mutation / genetics
  • Polymers / chemistry*
  • Serum Albumin, Bovine / chemistry
  • Serum Albumin, Bovine / metabolism*

Substances

  • Enzymes, Immobilized
  • Polymers
  • Serum Albumin, Bovine
  • Lipase