Use of the housekeeping genes, gdh (zwf) and gki, in multilocus sequence typing to differentiate Streptococcus pneumoniae from Streptococcus mitis and Streptococcus oralis

Margaret Ip; Fang Chi; Shirley S L Chau; Mamie Hui; Julian Tang; Paul K S Chan

doi:10.1016/j.diagmicrobio.2006.04.013

Use of the housekeeping genes, gdh (zwf) and gki, in multilocus sequence typing to differentiate Streptococcus pneumoniae from Streptococcus mitis and Streptococcus oralis

Diagn Microbiol Infect Dis. 2006 Nov;56(3):321-4. doi: 10.1016/j.diagmicrobio.2006.04.013. Epub 2006 Jun 12.

Authors

Margaret Ip¹, Fang Chi, Shirley S L Chau, Mamie Hui, Julian Tang, Paul K S Chan

Affiliation

¹ Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong. margaretip@cuhk.edu.hk

PMID: 16765553
DOI: 10.1016/j.diagmicrobio.2006.04.013

Abstract

Polymerase chain reaction and sequencing of the housekeeping genes, gdh (zwf) and gki, based on the primers and alleles from multilocus sequence typing can be used to delineate and support the identity of clinical isolates of Streptococcus pneumoniae and differentiate from the closely related Streptococcus mitis and Streptococcus oralis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques
Genes, Bacterial*
Phylogeny
Streptococcus mitis / genetics
Streptococcus mitis / isolation & purification*
Streptococcus oralis / genetics
Streptococcus oralis / isolation & purification*
Streptococcus pneumoniae / genetics
Streptococcus pneumoniae / isolation & purification*
Sugar Alcohol Dehydrogenases / genetics*
Sugar Alcohol Dehydrogenases / isolation & purification

Substances

Sugar Alcohol Dehydrogenases
galactitol 2-dehydrogenase

Grants and funding

Wellcome Trust/United Kingdom