Electrochemical investigation of immobilized hemoglobin: redox chemistry and enzymatic catalysis

Hui-Hong Liu; Guo-Lin Zou

doi:10.1016/j.jbbm.2006.04.001

Electrochemical investigation of immobilized hemoglobin: redox chemistry and enzymatic catalysis

J Biochem Biophys Methods. 2006 Aug 31;68(2):87-99. doi: 10.1016/j.jbbm.2006.04.001. Epub 2006 Apr 22.

Authors

Hui-Hong Liu¹, Guo-Lin Zou

Affiliation

¹ State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China. liuhh@xfu.edu.cn

PMID: 16762418
DOI: 10.1016/j.jbbm.2006.04.001

Abstract

Hb entrapped in the Konjak glucomannan (KGM) film could transfer electrons directly to an edge-plane pyrolytic graphite (EPG) electrode, corresponding to the redox couple of Fe(III)/Fe(II). The redox properties of Hb, such as formal potential, electron transfer rate constant, the stability of the redox state of protein and redox Bohr effect, were characterized by cyclic voltammetry and square wave voltammetry. The stable Hb-KGM/EPG gave analytically useful electrochemical catalytic responses to oxygen, hydrogen peroxide and nitrite.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biosensing Techniques*
Catalysis
Cattle
Electrochemistry / methods
Electrodes
Hemoglobins / chemistry*
Iron / chemistry*
Nitrites / analysis*
Oxidation-Reduction
Oxygen / analysis*

Substances

Hemoglobins
Nitrites
Iron
Oxygen