Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants

Clin Vaccine Immunol. 2006 Jun;13(6):704-7. doi: 10.1128/CVI.00417-05.

Abstract

We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Anticoagulants / pharmacology*
  • Antigens, CD / blood*
  • Antigens, CD / genetics
  • Antigens, Differentiation, Myelomonocytic / blood*
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Blood Cells / drug effects*
  • Blood Cells / metabolism
  • CD36 Antigens / blood*
  • CD36 Antigens / genetics
  • Cell Count / methods
  • Female
  • Flow Cytometry / methods
  • Humans
  • Male
  • Middle Aged
  • Monocytes / drug effects*
  • Monocytes / metabolism
  • Receptors, Cell Surface / blood*
  • Receptors, Cell Surface / genetics

Substances

  • Anticoagulants
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD163 antigen
  • CD36 Antigens
  • Receptors, Cell Surface