Abstract
The full-size genomes of 2 highly pathogenetic avian influenza (HPAI) virus strains isolated from a wild great-crested grebe (A/Grebe/Novosibirsk/29/05) and a domestic duck (A/Duck/Novosibirsk/56/05) in the tract of the Chany hollow, Barabino forest-steppe (Novosibirsk Region) during the epizootic outbreak in the summer of 2005. The reproductive properties of these strains successively increase in the series of cell lines BHK-2 --> LEH --> Vero-E6 --> MDCK --> PS. A/Grebe/Novosibirsk/29/05 and A/Duck/Novosibirsk/56/05 were shown to be genetically close in all genomic segments to both each other and a group of HPAI/H5N1 A/Qinghai 05 strains isolated from wild birds on the Kukunor Lake in the northwestern province of Tsinkhai, China, in May 2005. All the above strains have the HPAI/H5-specific amino acid sequence of a proteolytic cleavage site (PQGERRRKKRGLF) with Lys-627 in the protein PB2 (which is associated with increased virulence to mammalian cells), Glu-92 in the protein NS1 (that suppresses an antiviral response in the host), Ser-31 in M2 (that is a marker of rimantadine/amantadine sensitivity), 20-member amino acid deletion in the protein NA (positions 49-68) that is a marker of affiliation to the so-called genotype Z and of increased tropism to poultry.
MeSH terms
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Amantadine / pharmacology
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Amino Acid Substitution
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Animals
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Animals, Domestic / virology
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Animals, Wild / virology
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Antiviral Agents / pharmacology
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Birds / virology
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Cell Line
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Chlorocebus aethiops
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Cricetinae
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Disease Outbreaks*
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Dogs
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Genome, Viral*
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Humans
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Influenza A Virus, H5N1 Subtype / drug effects
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Influenza A Virus, H5N1 Subtype / genetics*
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Influenza A Virus, H5N1 Subtype / immunology
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Influenza A Virus, H5N1 Subtype / pathogenicity
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Influenza in Birds / epidemiology
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Influenza in Birds / virology*
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Kidney
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Peptide Hydrolases / genetics
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RNA-Dependent RNA Polymerase / genetics
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Rimantadine / pharmacology
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Siberia / epidemiology
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Species Specificity
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Swine
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Viral Matrix Proteins / genetics
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Viral Matrix Proteins / physiology
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Viral Nonstructural Proteins / genetics
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Viral Proteins / genetics
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Virulence / genetics
Substances
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Antiviral Agents
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M2 protein, Influenza A virus
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PA protein, influenza viruses
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Viral Matrix Proteins
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Viral Nonstructural Proteins
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Viral Proteins
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Rimantadine
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Amantadine
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RNA-Dependent RNA Polymerase
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Peptide Hydrolases
Associated data
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GENBANK/DQ230521
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GENBANK/DQ232605
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GENBANK/DQ232606
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GENBANK/DQ232607
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GENBANK/DQ232608
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GENBANK/DQ232609
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GENBANK/DQ232610
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GENBANK/DQ234073
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GENBANK/DQ234074
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GENBANK/DQ234075
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GENBANK/DQ234076
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GENBANK/DQ234077
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GENBANK/DQ234078